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human il1β/il1f2 duoset elisa  (R&D Systems)


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    R&D Systems human il1β/il1f2 duoset elisa
    List of primers used for qRT-PCR with their optimum annealing temperature.
    Human Il1β/Il1f2 Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il1%CE%B2+il1f2+duoset+elisa/m+csf/pmc08304967-168-0-4
    Average 90 stars, based on 1 article reviews
    human il1β/il1f2 duoset elisa - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Aggregated Tau-PHF6 (VQIVYK) Potentiates NLRP3 Inflammasome Expression and Autophagy in Human Microglial Cells"

    Article Title: Aggregated Tau-PHF6 (VQIVYK) Potentiates NLRP3 Inflammasome Expression and Autophagy in Human Microglial Cells

    Journal: Cells

    doi: 10.3390/cells10071652

    List of primers used for qRT-PCR with their optimum annealing temperature.
    Figure Legend Snippet: List of primers used for qRT-PCR with their optimum annealing temperature.

    Techniques Used: Sequencing

    List of antibodies used for Western blot.
    Figure Legend Snippet: List of antibodies used for Western blot.

    Techniques Used: Western Blot

    Aggregated PHF6 induced upregulation of IL1β mRNA and protein in HMC3 cells after 6 h. ( a ) An increase in the mRNA levels of IL1B was observed after 20 μM aPHF6 treatment in comparison to PHF6 treatment or untreated controls as quantified by qRT-PCR. ( b , c ) Densitometric analysis of Western blots confirmed the increased IL1β protein levels in HMC3 cells at 6 h. ( d ) No changes in IL1B mRNA levels among different groups were detected after 24 h. ( e , f ). Western blot analysis reflected similar IL1β protein levels in all groups. */# p < 0.05.
    Figure Legend Snippet: Aggregated PHF6 induced upregulation of IL1β mRNA and protein in HMC3 cells after 6 h. ( a ) An increase in the mRNA levels of IL1B was observed after 20 μM aPHF6 treatment in comparison to PHF6 treatment or untreated controls as quantified by qRT-PCR. ( b , c ) Densitometric analysis of Western blots confirmed the increased IL1β protein levels in HMC3 cells at 6 h. ( d ) No changes in IL1B mRNA levels among different groups were detected after 24 h. ( e , f ). Western blot analysis reflected similar IL1β protein levels in all groups. */# p < 0.05.

    Techniques Used: Comparison, Quantitative RT-PCR, Western Blot

    IL1β analysis after 3 h pre-treatment with Nigericin or LPS followed by 6 h stimulation with PHF6/aPHF6. The treatment with unaggregated PHF6 or aggregated aPHF6 lead to no secretion of IL1β. LPS was used as a positive control, which also lead to no secretion. For NLRP3 priming, cells were pre-treated with Nigericin or LPS for 3 h followed by PHF6 or aPHF6 treatment for 6 h. Only the combination of Nigericin and LPS stimulation increased the IL1β secretion into the supernatant. Only minor levels were found after Nigericin only, and in combination with aPHF6 or PHF6. Similar levels were found after LPS pre-treatment as a priming agent.
    Figure Legend Snippet: IL1β analysis after 3 h pre-treatment with Nigericin or LPS followed by 6 h stimulation with PHF6/aPHF6. The treatment with unaggregated PHF6 or aggregated aPHF6 lead to no secretion of IL1β. LPS was used as a positive control, which also lead to no secretion. For NLRP3 priming, cells were pre-treated with Nigericin or LPS for 3 h followed by PHF6 or aPHF6 treatment for 6 h. Only the combination of Nigericin and LPS stimulation increased the IL1β secretion into the supernatant. Only minor levels were found after Nigericin only, and in combination with aPHF6 or PHF6. Similar levels were found after LPS pre-treatment as a priming agent.

    Techniques Used: Positive Control

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Novel TMEM173 Mutation and the Role of Disease Modifying Alleles.
    Article Snippet: After a minimum of 3 h rest in complete RPMI 1640 (Lonza, Basel, Switzerland) cells were stimulated with 1μg/ml lipopolysaccharides (LPS) from E.coli O111:B4 (Sigma), 1μg/ml Pam3Cys-SKKKK (Pam3Cys) (EMC microcollections), and 5mM ATP (stock solution neutralized; Sigma) for the indicated times. .. TNF-α and the mature, cleaved forms of IL1β and IL18 were detected from PBMC culture media supernatants using Human TNF-α DuoSet ELISA, Human IL1β/IL1F2 DuoSet ELISA, and Human Total IL18 DuoSet ELISA (all from R&D Systems). .. PBMC RNA was isolated using the RNeasy Plus Mini Kit (Qiagen), followed by cDNA synthesis with iScript kit (Bio-Rad).



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    human il1β/il1f2 duoset elisa  (R&D Systems)
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    R&D Systems human il1β/il1f2 duoset elisa
    List of primers used for qRT-PCR with their optimum annealing temperature.
    Human Il1β/Il1f2 Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    List of primers used for qRT-PCR with their optimum annealing temperature.
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    List of primers used for qRT-PCR with their optimum annealing temperature.

    Journal: Cells

    Article Title: Aggregated Tau-PHF6 (VQIVYK) Potentiates NLRP3 Inflammasome Expression and Autophagy in Human Microglial Cells

    doi: 10.3390/cells10071652

    Figure Lengend Snippet: List of primers used for qRT-PCR with their optimum annealing temperature.

    Article Snippet: Human IL1β/IL1F2 DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) was performed as per the manufacturer’s protocol.

    Techniques: Sequencing

    List of antibodies used for Western blot.

    Journal: Cells

    Article Title: Aggregated Tau-PHF6 (VQIVYK) Potentiates NLRP3 Inflammasome Expression and Autophagy in Human Microglial Cells

    doi: 10.3390/cells10071652

    Figure Lengend Snippet: List of antibodies used for Western blot.

    Article Snippet: Human IL1β/IL1F2 DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) was performed as per the manufacturer’s protocol.

    Techniques: Western Blot

    Aggregated PHF6 induced upregulation of IL1β mRNA and protein in HMC3 cells after 6 h. ( a ) An increase in the mRNA levels of IL1B was observed after 20 μM aPHF6 treatment in comparison to PHF6 treatment or untreated controls as quantified by qRT-PCR. ( b , c ) Densitometric analysis of Western blots confirmed the increased IL1β protein levels in HMC3 cells at 6 h. ( d ) No changes in IL1B mRNA levels among different groups were detected after 24 h. ( e , f ). Western blot analysis reflected similar IL1β protein levels in all groups. */# p < 0.05.

    Journal: Cells

    Article Title: Aggregated Tau-PHF6 (VQIVYK) Potentiates NLRP3 Inflammasome Expression and Autophagy in Human Microglial Cells

    doi: 10.3390/cells10071652

    Figure Lengend Snippet: Aggregated PHF6 induced upregulation of IL1β mRNA and protein in HMC3 cells after 6 h. ( a ) An increase in the mRNA levels of IL1B was observed after 20 μM aPHF6 treatment in comparison to PHF6 treatment or untreated controls as quantified by qRT-PCR. ( b , c ) Densitometric analysis of Western blots confirmed the increased IL1β protein levels in HMC3 cells at 6 h. ( d ) No changes in IL1B mRNA levels among different groups were detected after 24 h. ( e , f ). Western blot analysis reflected similar IL1β protein levels in all groups. */# p < 0.05.

    Article Snippet: Human IL1β/IL1F2 DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) was performed as per the manufacturer’s protocol.

    Techniques: Comparison, Quantitative RT-PCR, Western Blot

    IL1β analysis after 3 h pre-treatment with Nigericin or LPS followed by 6 h stimulation with PHF6/aPHF6. The treatment with unaggregated PHF6 or aggregated aPHF6 lead to no secretion of IL1β. LPS was used as a positive control, which also lead to no secretion. For NLRP3 priming, cells were pre-treated with Nigericin or LPS for 3 h followed by PHF6 or aPHF6 treatment for 6 h. Only the combination of Nigericin and LPS stimulation increased the IL1β secretion into the supernatant. Only minor levels were found after Nigericin only, and in combination with aPHF6 or PHF6. Similar levels were found after LPS pre-treatment as a priming agent.

    Journal: Cells

    Article Title: Aggregated Tau-PHF6 (VQIVYK) Potentiates NLRP3 Inflammasome Expression and Autophagy in Human Microglial Cells

    doi: 10.3390/cells10071652

    Figure Lengend Snippet: IL1β analysis after 3 h pre-treatment with Nigericin or LPS followed by 6 h stimulation with PHF6/aPHF6. The treatment with unaggregated PHF6 or aggregated aPHF6 lead to no secretion of IL1β. LPS was used as a positive control, which also lead to no secretion. For NLRP3 priming, cells were pre-treated with Nigericin or LPS for 3 h followed by PHF6 or aPHF6 treatment for 6 h. Only the combination of Nigericin and LPS stimulation increased the IL1β secretion into the supernatant. Only minor levels were found after Nigericin only, and in combination with aPHF6 or PHF6. Similar levels were found after LPS pre-treatment as a priming agent.

    Article Snippet: Human IL1β/IL1F2 DuoSet ELISA (R&D Systems, Minneapolis, MN, USA) was performed as per the manufacturer’s protocol.

    Techniques: Positive Control